Variation in the extent of sex chromosome differentiation exists within the same population for the common frog, Rana temporaria. This alows to directly compare possible effects on male fitness while controlling for the rest of the genome, and the environment.
RNAseq analysis of males and females from a population with varying degrees of genetic differentiation between the X and the Y chromosomes.
Sequencing of only the Y chromosome is possible through the generation of haploid embryos in frogs.
Gene expression in experimentally evolved D. pseudoobscura lines under high and low sexual selection.
Selection and QTL analysis in within-population crosses of D. montana.