Confirmation of male-specific PCR markers in the annual plant Mercurialis annua.
Bacterial artificial chromosome (BACs) containing a Y-specific marker has been sequenced with PacBio technology. The assembled sequence has been annotated. I am in the process of sequencing exons and introns from the predicted genes in an attempt to identify sex-specific PCR products and characterise sequences from the X and Y chromosomes.
The long term goal is to identify the sex-determining gene, which may require chromosome walking through the BAC library.
Another aim is to obtain sequences from the closely related polyploid lineages/species that have males (M. huetti and M. canariensis) to determine whether they share the same sex-determining region.