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For Academics

Worm protocols

Collection of protocols and buffers for use with C. elegans.

Lexogen Quantseq library prep

Protocol to prepare 3' RNA libraries. Modified from the Lexogen protocol to use 1/4 reactions.

The numbers in gray refer to electronic repetitive pipetting.

The elutions are in larger volumes than 1/4th.

The first step could use less concentrated RNA samples, so that water is not needed. The Qiagen Plant RNA extraction kit elution volume of 60 µl should be sufficient with typical Mimulus leaf extractions.

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Sex chromosome papers RSS


The genome sequence of the common hop, Humulus lupulus L
Link

Rare recombination in Salix nivalis and the maintenance of homomorphic sex chromosomes in willows
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The heterogametic transition in European Bufo toads switches the sex-linkage of key vertebrate sex-determination genes and associates with a large sex-chromosome-effect
Link