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For Academics

Lexogen Quantseq library prep

Protocol to prepare 3' RNA libraries. Modified from the Lexogen protocol to use 1/4 reactions.

The numbers in gray refer to electronic repetitive pipetting.

The elutions are in larger volumes than 1/4th.

The first step could use less concentrated RNA samples, so that water is not needed. The Qiagen Plant RNA extraction kit elution volume of 60 ┬Ál should be sufficient with typical Mimulus leaf extractions.

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Sex chromosome twitter bot

Mimulus twitter bot

Silene twitter bot

I setup the sexChr_papers twitter bot following Rob Lanfear's instructions.

I am interested in papers on sex chromosome evolution, and thus remove medical search terms. The pubmed search is sex chromosome NOT aneuploidy NOT patient? NOT chronic NOT acute NOT clinical NOT prenatal NOT diagnostic NOT congenital NOT infant? NOT syndrome NOT trisom* NOT sperm.

Casey Bergman is gathering literature Twitter bots here.