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For Academics

Worm protocols

Collection of protocols and buffers for use with C. elegans.

Lexogen Quantseq library prep

Protocol to prepare 3' RNA libraries. Modified from the Lexogen protocol to use 1/4 reactions.

The numbers in gray refer to electronic repetitive pipetting.

The elutions are in larger volumes than 1/4th.

The first step could use less concentrated RNA samples, so that water is not needed. The Qiagen Plant RNA extraction kit elution volume of 60 µl should be sufficient with typical Mimulus leaf extractions.

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Sex chromosome papers RSS

The genome sequence of the Winter Shade, Tortricodes alternella [Denis & Schiffermüller], 1775

Whole-genome DNA methylomes of Tree shrew brains reveal conserved and divergent roles of DNA methylation on sex chromosome regulation

The genome sequence of a hoverfly, Syrphus vitripennis (Meigen, 1822)