Swift 2S Turbo DNA library quarter version
High throughput Drosophila DNA extraction
Lexogen Quantseq library prep
Protocol to prepare 3' RNA libraries. Modified from the Lexogen protocol to use 1/4 reactions.
The numbers in gray refer to electronic repetitive pipetting.
The elutions are in larger volumes than 1/4th.
The first step could use less concentrated RNA samples, so that water is not needed. The Qiagen Plant RNA extraction kit elution volume of 60 µl should be sufficient with typical Mimulus leaf extractions.
Qiagen Mimulus DNA extraction
High molecular weight plant DNA extraction
Qiagen Mimulus RNA extraction
Cluster usage tips for Mac.
Standardised project folder structure
Define and open a standardised project structure in the Finder, to remove mental load in switching between projects.
Mimulus twitter bot
Silene twitter bot
I setup the sexChr_papers twitter bot following Rob Lanfear's instructions.
I am interested in papers on sex chromosome evolution, and thus remove medical search terms. The pubmed search is sex chromosome NOT aneuploidy NOT patient? NOT chronic NOT acute NOT clinical NOT prenatal NOT diagnostic NOT congenital NOT infant? NOT syndrome NOT trisom* NOT sperm.
Casey Bergman is gathering literature Twitter bots here.