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Qubit DNA quantification

Notes on using Qubit.

  1. Prepare N tubes + 2 for CS1 and CS2
  2. Prepare master mix (MM)

    N tubes + 2 (CS1, CS2) + 10%

    Solution 1x (μl)
    Buffer 199
    Fluorochrome 1
    Final volume 200

    Vortex well and use within 1 hr.

  3. Prepare the tubes

    CS1: 190 ul (MM) + 10 μl (CS1) CS2: 190 ul (MM) + 10 μl (CS2) Samples: 198 ul (MM) + 2 μl (DNA)

    Vortex well and centrifuge the tubes and let stand for 5 min.

  4. Use Qubit

    Select 'Quant IT dsDNA HS'

    1. Put CS1 and read
    2. Put CS2 and read
    3. Keep CS2 and 'sample' > 'calculate sample concentration' > '10 μl' > 'ng/μl'
    4. Add the first sample 'read' > 'calculate sample concentration' > '2 μl' > 'ng/μl'

    Remember to remove the last stample from the machine.

    Record your usage in the log.

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